Administrator KPMG was appointed late last week and all the staff bar one were made redundant.
The Scottish Government said it is making “every effort to support workers” in the town of Dunoon where McNee was based and was a major local employer.
Business Minister Fergus Ewing said: “KPMG have confirmed that they have made 60 employees of Stuart McNee construction redundant, most of whom live in the town.
“This news will be devastating for employees, their families and the town of Dunoon and the Scottish Government will do everything possible to help.
“Our initiative for responding to redundancy situations, Partnership Action for Continuing Employment (PACE) is already engaged with the administrators, who will today issue a letter on our behalf inviting employees to attend four PACE drop in sessions over the next fortnight to provide immediate help.
“Through providing skills development and employability support, PACE aims to minimise the time that individuals affected by redundancy are out of work. Skills Development Scotland will also help the five Modern Apprentices who trained at the company.”
KPMG said the firm “succumbed to market and cash flow pressures” and they are now looking to transfer ongoing schemes to other contractors.
The firm had been in business since 1973