Leaflets have been distributed to union activists across the country calling for people to protest at the Hackney Wick site entrance in east London.
The protesters claim blacklists are still in use on construction sites despite the high-profile investigation into the practice in 2009.
The demonstrators are also highlighting the plight of 400 construction workers in dispute with Redhall Engineering at the BP plant at Saltend near Hull.
A publicity leaflet for the protest states: “The Olympics is the most high-profile construction project in the world.
“Any action at the Olympics will be headline news. Let’s show the construction firms that we got some self-respect.”