Construction union Ucatt promised a “huge amount of disruption” as 180 drivers from HTC Plant Limited start three days of industrial action.
Stoppages have been called for three successive Fridays in November on the 7th, 14th and 21st following a dispute over pay.
Contractors including Kier, Lend Lease, Bam, Costain, Mace and Vinci are likely to be affected.
Sites hit will include Crossrail, London Bridge, Nova Square Victoria, Elephant and Castle and the Atomic Weapons Establishment at Aldermarston.
Steve Murphy, General Secretary of Ucatt, said: “Construction sites are facing massive disruption because HTC have failed to make a realistic pay offer to our members.”