The training body has been in business since 2011 working with major contractors and clients across the country.
The Centre of Refurbishment Excellence confirmed that retrofit training courses have now stopped and all staff have been released.
The organisation worked in partnership with BRE, Stoke-on-Trent City Council, Federation of Master Builders and the National Energy Foundation.
CoRE Ltd, a partnership between Stoke-on-Trent City Council, the Building Research Establishment and private industry, offered courses in eco-friendly retrofitting techniques.
The council is understood to have turned down requests for further funding.
The organisations main building will continue to operate as an events venue managed directly by the council.Deputy council leader Abi Brown told the Stoke Sentinel: “The council was asked to provide additional financial support to CoRE as we have done in the past due to severe financial pressures to keep the business operating.
“We are simply not in a position to continue to do this, particularly as we are now aware of the full extent of additional support required.
“The council continues to work closely with central government to ensure the building is used as required by the original grant funding.
We have acted quickly to protect the interests of the taxpayers and ensure that this landmark building remains open.”
CoRE Ltd had 43 member companies at the time it ceased trading, each paying up to £600 a year to access training courses and events.