The move follows a string of incidents where motorists have ignored the stop signs.
County Councillor David Nimmo Smith, Cabinet Member for Transport, said: “Oxfordshire County Council takes staff safety and any abuse of its employees and subcontractors very seriously and any actions that put road workers at risk will not be tolerated.
“All we ask is that people have consideration and understanding that they are just doing their jobs and abusive behaviour is not acceptable.”
Richard Lovewell, Operations Manager at Skanska, said: “There was an incident where an operative was hit by a car that ignored the stop sign.
“Thankfully he walked away with minor injuries, but it could have been a lot worse. There have also been incidents of motorists verbally abusing workers.
“These signs will work as an effective deterrent and encourage drivers to slow down and pay attention, improving the safety of our workers and the travelling public.”
The signs were developed and manufactured by Eurosigns.
They are equipped with a wide-angle, automatic recording system, providing hi-resolution images.
Chris Appleby, Casualty Reduction Sergeant, Joint Operations Unit, Hampshire Constabulary and Thames Valley Police, explained: “Video footage of drivers breaking the law can be very helpful in identifying drivers and gaining successful prosecutions.”