The Generation 3 framework deal has been organised by Hampshire County Council and will cover projects valued between £8m to £25m.
It lasts four years and can be used by over 40 local authorities spanning an area from Devon to Kent.
The three winners are Bam Nuttall, Carillion and Skanska.
Councillor Seán Woodward, Executive Member for Economy, Transport and Environment at Hampshire County Council, said: “Hampshire is leading the way here in establishing this framework for the whole of the South.
“Good infrastructure is key to the economic prosperity of any area, and this Generation 3 Framework will benefit not only the local economy but all who live and work in the area by ensuring resources are well used and money is well spent.
“The framework will support our own ambitious capital programme of works, and shows the importance of scale and collaboration, saving us, and all the authorities in the South, time and money by not having to procure separately each time we get the go-ahead for a major project.
“We’ve assessed these suppliers thoroughly on everything from performance and value for money through to their sustainable policies and commitment to apprenticeships, with an emphasis on public service delivery.”