Technical problems on Sunday saw people trapped at ground level due to problems with door sensors.
And last Thursday passengers were stuck in mid-air inside the tower’s observation pod which travels up to a full height of 137 metres.
The i360 is the world’s thinnest tall building and opened last month.
Passengers ascend the tower in a 360-degree curved glass pod.
The doughnut-shaped main observation pod was built by French cable-car specialist POMA, which delivered the London Eye pods.
The £46.2m scheme was designed by Marks Barfield Architects and Jacobs.
West Sussex contractor Mackley was in charge of the civils package on the project while Dutch steelwork specialist Hollandia erected the main steel mast.
A British Airways statement said: “British Airways i360 will remain closed tomorrow [September 13] while checks continue.
“We advise customers to visit our website and social media channels for updates.
“We apologise for any inconvenience caused and will continue to contact customers who have already made bookings to offer them the chance to rebook or claim a refund.”